Characterization of virus-like particles associated with the human faecal and caecal microbiota.
نویسندگان
چکیده
This work represents an investigation into the presence, abundance and diversity of virus-like particles (VLPs) associated with human faecal and caecal samples. Various methodologies for the recovery of VLPs from faeces were tested and optimized, including successful down-stream processing of such samples for the purpose of an in-depth electron microscopic analysis, pulsed-field gel electrophoresis and efficient DNA recovery. The applicability of the developed VLP characterization method beyond the use of faecal samples was then verified using samples obtained from human caecal fluid.
منابع مشابه
Characterization of virus - like particles associated with the human faecal and caecal 1 microbiota 2 3
24 This work represents an investigation into the presence, abundance and diversity of 25 virus-like particles (VLPs) associated with human faecal and caecal samples. Various 26 methodologies for the recovery of VLPs from faeces were tested and optimized, including 27 successful down-stream processing of such samples for the purpose of an in-depth electron 28 microscopic analysis, pulsed-field ...
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عنوان ژورنال:
- Research in microbiology
دوره 165 10 شماره
صفحات -
تاریخ انتشار 2014